glucose transporter type 4 glut 4 level Search Results


gene exp slc2a4 hs00168966 m1  (Thermo Fisher)
95
Thermo Fisher gene exp slc2a4 hs00168966 m1
Gene Exp Slc2a4 Hs00168966 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucose transporter type 4  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc glucose transporter type 4
Glucose Transporter Type 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucose transporter type 4  (Thermo Fisher)
99
Thermo Fisher glucose transporter type 4
Glucose Transporter Type 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti glucose transporter type 4 glut4  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti glucose transporter type 4 glut4
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Anti Glucose Transporter Type 4 Glut4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glut4 surface expression levels  (MedChemExpress)
92
MedChemExpress glut4 surface expression levels
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Glut4 Surface Expression Levels, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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antibodies against glucose transporter type 4  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology antibodies against glucose transporter type 4
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Antibodies Against Glucose Transporter Type 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/Glut4/pmc11795245-41-45-78
Average 95 stars, based on 1 article reviews
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serum glut4 levels  (Shanghai Korain Biotech Co Ltd)
94
Shanghai Korain Biotech Co Ltd serum glut4 levels
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Serum Glut4 Levels, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/Rat+Glucose+Transporter+4/10__1155_slash_jnme_slash_2432900-73-0-13
Average 94 stars, based on 1 article reviews
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anti-glut-4 (glucose transporter type 4)  (Merck KGaA)
90
Merck KGaA anti-glut-4 (glucose transporter type 4)
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Anti Glut 4 (Glucose Transporter Type 4), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/antibodies+against+glut4/pmc10143944-25-0-7
Average 90 stars, based on 1 article reviews
anti-glut-4 (glucose transporter type 4) - by Bioz Stars, 2026-10
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glut4  (Absolute Biotech Inc)
86
Absolute Biotech Inc glut4
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Glut4, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/glut4/miwa_hiromi__2022__optimization_of_particle_based_hydrogel_biophysical_and_biochemical_properties_for_biomedical_applications-413-48-53
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glut 4 protein band  (Bio-Rad)
99
Bio-Rad glut 4 protein band
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Glut 4 Protein Band, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/Protein+Standard+I/pm10720068-78-2-12
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anti glut 4  (ABclonal Biotechnology)
95
ABclonal Biotechnology anti glut 4
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Anti Glut 4, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+transporter+type+4+glut+4+level/GLUT4+Rabbit+pAb/pm37109458-336-77-74
Average 95 stars, based on 1 article reviews
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gene exp slc2a4 mm01245502 m1  (Thermo Fisher)
98
Thermo Fisher gene exp slc2a4 mm01245502 m1
Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, <t>GLUT4</t> and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.
Gene Exp Slc2a4 Mm01245502 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, GLUT4 and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.

Journal: Journal of Endocrinology

Article Title: INT-767 prevents NASH and promotes visceral fat brown adipogenesis and mitochondrial function

doi: 10.1530/joe-17-0557

Figure Lengend Snippet: Figure 4 Effects of INT-767 treatment on adipocyte size, hypoxia, GLUT4 and RhoA membrane translocation and UCP1 in VAT sections from RD, HFD and HFD plus INT-767-treated rabbits. Panel A shows the histomorphometric analysis of adipocyte diameter (µm) in the different experimental groups (n = 3/group) with hematoxylin-eosin staining (scale bar = 100 µm). Panel B shows the immunohistochemical staining of hypoxyprobe adducts in VAT sections. Hypoxyprobe adducts were revealed in hypoxic cells (PO2 <10 mmHg) of VAT transverse sections by a hypoxyprobe-1-MAb (scale bar = 100 µm). RD optical density was taken as 100% in the computer-assisted quantitative image analysis of three independent experiments for each group. Panel C shows representative immunoblots with anti-GLUT4 and anti-signal transducer and activator of transcription 1 (STAT1) primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values. Panel D shows representative immunoblots with anti-RhoA and anti-actin primary antibodies on membrane (m) and cytosolic (c) VAT protein extracts. Bar graphs show the optical density analyses of membrane/cytosol RhoA ratio (n = 3/group). Data are expressed as percentage of RD values. Panel E shows representative immunoblots with anti-UCP1 and anti-STAT1 primary antibodies on VAT protein extracts from experimental rabbits. Bar graphs show the optical density analyses of UCP1 (n = 3/group). Data are expressed as percentage of RD values (°P < 0.05, °°P < 0.001, °°°P < 0.0001 vs RD; *P < 0.01, **P < 0.001, ***P < 0.0001 vs HFD). A full colour version of this figure can be found at https://doi.org/10.1530/ JOE-17-0557.

Article Snippet: Western blot analysis with an anti-glucose transporter type 4 (GLUT4) antibody (1:500 vol/vol; Upstate Biotechnology, Lake Placid, NY, USA), anti-ras homolog member A (RhoA) (1:500 vol/vol; Santa Cruz Biotechnology) and anti-theromogenin (UCP1) antibody (1:200 vol/vol; Santa Cruz Biotechnology) was performed as previously described (Maneschi et al. 2016). https://doi.org/10.1530/JOE-17-0557 http://joe.endocrinology-journals.org © 2018 Society for Endocrinology Published by Bioscientifica Ltd.

Techniques: Membrane, Translocation Assay, Staining, Immunohistochemical staining, Western Blot

Figure 5 INT-767 treatment modulates mRNA expression of target genes in rabbit visceral fat (A) and liver (B and C) tissues. Data are calculated per the 2−ΔΔCt comparative method, using the 18S ribosomal RNA subunit as reference gene for normalization and are reported as fold-change of mRNA expression over RD. Statistical analysis was performed using Kruskal–Wallis and Mann–Whitney tests (°P < 0.05, °°P < 0.01, °°°P < 0.001 vs RD; *P < 0.05, **P < 0.01, ***P < 0.001 vs HFD). Panel D shows representative immunoblots with anti-GLUT4 and anti-STAT1 primary antibodies on membrane (m) and cytosolic (c) liver protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values (°P < 0.01 vs RD; *P < 0.05 vs HFD).

Journal: Journal of Endocrinology

Article Title: INT-767 prevents NASH and promotes visceral fat brown adipogenesis and mitochondrial function

doi: 10.1530/joe-17-0557

Figure Lengend Snippet: Figure 5 INT-767 treatment modulates mRNA expression of target genes in rabbit visceral fat (A) and liver (B and C) tissues. Data are calculated per the 2−ΔΔCt comparative method, using the 18S ribosomal RNA subunit as reference gene for normalization and are reported as fold-change of mRNA expression over RD. Statistical analysis was performed using Kruskal–Wallis and Mann–Whitney tests (°P < 0.05, °°P < 0.01, °°°P < 0.001 vs RD; *P < 0.05, **P < 0.01, ***P < 0.001 vs HFD). Panel D shows representative immunoblots with anti-GLUT4 and anti-STAT1 primary antibodies on membrane (m) and cytosolic (c) liver protein extracts. The bar graphs show the optical density analyses of membrane/cytosol GLUT4 ratio (n = 3/group). Data are expressed as percentage of RD values (°P < 0.01 vs RD; *P < 0.05 vs HFD).

Article Snippet: Western blot analysis with an anti-glucose transporter type 4 (GLUT4) antibody (1:500 vol/vol; Upstate Biotechnology, Lake Placid, NY, USA), anti-ras homolog member A (RhoA) (1:500 vol/vol; Santa Cruz Biotechnology) and anti-theromogenin (UCP1) antibody (1:200 vol/vol; Santa Cruz Biotechnology) was performed as previously described (Maneschi et al. 2016). https://doi.org/10.1530/JOE-17-0557 http://joe.endocrinology-journals.org © 2018 Society for Endocrinology Published by Bioscientifica Ltd.

Techniques: Expressing, MANN-WHITNEY, Western Blot, Membrane